A Primer on Metagenomics

A Primer on Metagenomics

Metagenomics is a discipline that enables the genomic study of uncultured microorganisms. Faster, cheaper sequencing technologies and the ability to sequence uncultured microbes sampled directly from their habitats are expanding and transforming our view of the microbial world. Distilling meaningful information from the millions of new genomic sequences presents a serious challenge to bioinform...

Better primer design for metagenomics applications by increasing taxonomic distinguishability

Current methods of understanding microbiome composition and structure rely on accurately estimating the number of distinct species and their relative abundance. Most of these methods require an efficient PCR whose forward and reverse primers bind well to the same, large number of identifiable species, and produce amplicons that are unique. It is therefore not surprising that currently used univ...

Beyond research: a primer for considerations on using viral metagenomics in the field and clinic

Powered by recent advances in next-generation sequencing technologies, metagenomics has already unveiled vast microbial biodiversity in a range of environments, and is increasingly being applied in clinics for difficult-to-diagnose cases. It can be tempting to suggest that metagenomics could be used as a "universal test" for all pathogens without the need to conduct lengthy serial testing using...

A PRIMER ON FUZZY OPTIMIZATION MODELS AND METHODS

Fuzzy Linear Programming models and methods has been one ofthe most and well studied topics inside the broad area of Soft Computing. Itsapplications as well as practical realizations can be found in all the real worldareas. In this paper a basic introduction to the main models and methods infuzzy mathematical programming, with special emphasis on those developedby the authors, is presented. As ...

A Primer on Bioethics